‘Sure, that is definitely about proper. I seek for a physical comprehension of Organic methods in the base-up way. I think you might distinguish a few traces of investigate in my group in the meanwhile. The first issues chromosome framework: How does one get a metre-extended DNA polymer folded and organised in this type of microscopic mobile? We have produced visualisation tactics that allow for us to study how, by way of example, SMC proteins loop DNA (movie on Youtube) to be able to Construct up chromosomes.
1996, initial mesoscopic cost density waves devices; and very first electrical measurements on only one metal nanocluster amongst nanoelectrodes
Multicolor imaging of the bacterial nucleoid and mobile division proteins with blue, orange, and in close proximity to-infrared fluorescent proteins
A rhythmically pulsing leaf-spring DNA-origami nanoengine that drives a passive follower Mathias Centola
This yielded a breakthrough. Within just only a few months, we had been able to evaluate transportation by way of an individual solitary-walled carbon nanotube. Based on lower-temperature experiments by a brilliant PhD college student in my group, Sander Tans (now a valued colleague and professor at Delft College of Engineering having an Energetic exploration lab at AMOLF in Amsterdam), we showed that carbon nanotubes ended up genuine quantum wires with extremely extensive (micrometres) electronic coherence lengths — confirming theoretical predictions of metallic behaviour.
Our exploration ranges through the biophysics of DNA to synthetic cells and outside of. Remember to take a look underneath in the spectrum of our present-day initiatives.
Several relevant developments adopted, where by our group set up distinct transistor variants for example solitary-electron transistors at area temperature and intramolecular nanotube junctions that acted as rectifying diodes, and coupled a number of transistors into smaller evidence-of-theory electronic circuits.
Case in point (1) of bending configurations in 3D simulations of DNA rods on nanopores. A part of the membrane is shown in gray, the rim of the pore is highlighted in purple, along with a 3D rendering with the motion with the DNA rod is displayed.
The rotors harness Vitality from a nanoscale h2o and ion circulation ceesdekker that's created by a static chemical or electrochemical likely gradient inside the nanopore, which are recognized by way of a salt gradient or used voltage, respectively. These artificial nanoengines self-Manage and run autonomously in physiological circumstances, suggesting tips on how to setting up energy-transducing motors at nanoscale interfaces.
There was a person difficulty while — we didn't notice any measurable electrical conduction from The one polymer molecules. We concluded that at The one-molecule amount, conducting polymers were being actually not such fantastic conductors, which was fairly disappointing. We Hence broadened our look at to try to search out additional promising molecules.
An built-in microfluidic System for quantifying drug permeation throughout biomimetic vesicle membranes
2006, initial demonstration of molecular sorting in the lab with a chip using biomotors; discovery of nanobubbles in sound-condition nanopores; and to start with estimate of electrokinetic Power conversion in the nanofluidic channel
Visualization of unstained DNA nanostructures with Superior in-emphasis transmission electron microscopy techniques
Solid-state nanopores have confirmed to become a astonishingly adaptable probe for solitary-molecule Examination of DNA. I will explain many of our the latest conclusions – exclusively DNA knots – and also our initiatives to increase the abilities of solid-point out nanopores even even further, within the route of single-protein detection, graphene nanopores, plasmonic nanopores, and DNA origami nanopores.